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At least 3 amino acid substitutions must be present for phenotypic resistance to indinavir to reach measurable levels. In general, higher levels of resistance were associated with the co-expression of greater numbers of substitutions, although their individual effects varied and were not additive. No single substitution was either necessary or sufficient for measurable resistance (≥4-fold increase in IC 95). Resistance was mediated by the co-expression of multiple and variable substitutions at these positions. Eleven amino acid residue positions, (L10l/V/R, K20l/M/R, L24l, M46l/L, l54A/V, L63P, l64V, A71T/V, V82A/F/T, l84V, and L90M), at which substitutions are associated with resistance, have been identified. Viral resistance was correlated with the accumulation of mutations that resulted in the expression of amino acid substitutions in the viral protease. Titanium 12 pro is the mDownload Roxio Toast 12 Titanium torrent from software category on Isohunt.Isolates of HIV-1 with reduced susceptibility to the drug have been recovered from some patients treated with indinavir.
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